用MPVⅡ型显微分光光度计测量乳腺单纯癌及乳腺腺病细胞核DNA含量及面积

THE DETERMINATION OF CELL一NUCLEAR DNA CONTENT AND IN AREASIMPLEX CARCINOMA OF BREAST AND MAMMARY ADENOSIS

摘要: 用MPVⅡ型显微分光光度计与HP—9825A微型计算机连机操作,测量了5例乳腺单纯癌和3例乳腺腺病的细胞核DNA含量和面积,同时,以淋巴细胞做为内插二倍体标准。测量数据经微机处理,可同时获得细胞核之DNA含量和核面积两个参数,井分别以数字同时显示其相对值。其DNA组方图显示明显的、不规则的非整倍体时,对鉴别恶性肿瘤性生长和良性病变是有意义的,但有些恶性肿瘤的组方图如№。1与良性病变相似,故应注意假阴性。

Abstract: Cell-nuclear DNA content and area of simplex MPV microspectro photometer linking up with HP-9825A microcomputer .10 lymphocytes in each peparation were used as initernal diploid standards.Disposing the measured by micro-computer,we canobtain and show two pares meters,cell nuclear DNA content and area at the same time.