Abstract: This article reported 47 cases of PHC with negative and low grade positive AFP.Once hepatic aspiration was performed percutaneously with a bigger inside diameter N.9.In the same time,the aspirated material was examined cytologically and histopathogically.By correlative observation of all these cases,positive diagnosis in once aspiration reached 97.7%.The overall rate of accuracy in this series was 100%.For the prevention of the complications,the author improved the structure of the puncture needle and offered some procedures to avoid the dispersion of cancer cells along the puncture route.