Abstract: Flow cytometry(FCM) and silver colloid staining technique have special valve for retrospective analyses of the tumors.The DNA ploidy and Ag-NOR counts in 67 paraffin-embeded samples were analyzed,5 or 7-year survival rate was 78.0%(32/41) and 39.0%(16/41) for the patients with DNA diploid tumor,while 26.9%(7/26) and 11.5%(3/26) for the patients with DNA non-diploid trmpr(p<0.O1 and p<0.05 respectively).The survival percentage at 5 or 7 yearswas 80.0%(28/35) and 40.0%(14/35) for the patients with Ag-NOR counts less thaw X±SD=6.9±1.O,while 34.4(11/32) and 15.6%(5/32) for those with Ag-NOR counts more than that(p<0.0l and p<0.05 respectively).DNA ploidy and Ag-NOR counts were closely related to prognostic factors of colonic cancer.DNA diploid was 97.7%(34/35) for the patients with Ag-NOR counts less than the mean of 6.9±1.O,while 21.9%(7/32) for the patients with Ag-NOR counts more than that(p<0.01).These findings suggest that DNA ploidy and Ag-NOR counts be considered as prognostic parameters of colonic cancer.