Abstract: Feulgen's staining and AgNOR silver staining were employed to detect the DNA content and AgNOR count. Our findings are as follows: DNA content Grade Ⅲ in thyroid cancer groupthat in atypical nodular groupthat in noduler goiter groupthat in contrast group, while Grade Ⅰ is the opposite; AgNOR average count of the thyroid cancer group (9. 27±2. 72) of the atypical nodular group (4. 78±1. 66) of the nodular goiter group (3. 16±1. 38) contrast group (3. 08±0. 41) ; there's marked difference between the thyroid cancer group and the other groups (P0.01) and there's markable difference among the atypical nodular group, the nodular goiter group and the contrast group (P0. 01) and there's no difference between the nodular goiter group and the contrast group (P0. 05). This study suggests the marked corelation of the average count of AgNOR and DNA content and a direct ratio with cellproliferation, therefore, it is of affirmative velve in differetiating benign, malignant carcinoma and junctional lesion.