Abstract: Scorpion Venom Crude Ⅱ(SVC Ⅱ)was one of the effective components which was isolated from the scorpion venom crude extracted from Buthus Martensii Karsch in Henan Province.The experimentalresults showed that SVC Ⅱ with the concentration ranging from 4μg/ml to 20μg/ml had obvious killing acticitv on the human rectum adenocarcinoma C1184 cell line in Vitro in comparison with the positive control which was Mitomycin-c.Compared with the blank control,SVC Ⅱcould induceto increase the inhibitary rate of cell growth,the cvtotoxicitv and inhibited rate of the colonv formation(P0.05 or P0.01).There existed the dose-response correlations between the inhibitioneffect and the dose of SVC Ⅱ.The half inhibition concentrations of cell growth(Ⅰ C50)in above experiments were 11.20μg/ml、11.93μg/ml and 17. 38μg/ml,respectively.It was found that SVCⅡ signficantly inhibited C1184 cell growth in the dose-dependent and time-dependent manner in cell growth curve.The mitotic index of C1184 cells could be reduced by SVC Ⅱ.So it was supported that.SVC Ⅱpossess antineoplastic effect on tumor cell proliferation.