卡铂对A549肺癌细胞的抗癌作用及其机制的初步探讨
The anticancer effects of carboplatin on A_(549) lung cancer cells and its mechanism
-
摘要: 本文观察了卡铂对A549肺癌细胞DNA合成、DNA链间交联的影响及细胞超微结构的改变。依卡铂剂量不同将实验分为A、B、C、D四组,剂量分别为20μg/ml、40μg/ml、60μg/ml、80μg/ml,另设空白对照E组。结果表明,卡铂可能是DNA模板损伤型药物,通过引起DNA交联而阻碍其复制,产生杀细胞效应。Abstract: In order to study the anticancer effects of carboplatin (CBDCA) in vitro.We have examined the inhibition of DNA synthesis,DNA interstrand cross-linking(ISC)and the changes of ultrastructure of A549 Lung cancer cells (A549 cells).Four experimental groups, named A (20ug/ml)B(40ug/ ml),C(60ug/ml) and D (80ug/ml)according to different dosage of CBDCA and one group without CBDCA as control were used. In conclusion,we demonstrate that CBDCA is a DNA breakage analog,The DNA ISC induced by CBDCA is related to the inhibition of DNA replication and cytotoxicity.
-
Key words:
- CBDCA /
- anticancer effect /
- A549 cell /
- DNA replication /
- DNA ISC
-
-
1. canetta R, et al. Carboplatin Current status and future prospect. Cancer Treat. Rep. 1988,15:17 2. Canetta R, et al. Carboplatin, the clinical spectrum to date. Cancer Treat. Rev. 1985,12:125 3. Ciard DJ, et al. In vitro cultivation of human tumor establishment of cell lines derived from a series of solid tumors. J. Nat. Cancer Inst, 1973,51:1417 4. De Jong S, et al. Detection oI DNA Cross-Links in fu-mor cells with the ethidium bromide fluorescence as-say. Int. J. Cancer, 1986,37:557 5. Schroyens W, et al. Comparative effect of cisplatin, spiroplatin, carboplatin and iproplatin in a human fu-mor clonogenic assay. J. Cancer Res. Clin Oncol, 1990,116:392 6. Knox RJ, et al. Mechanism of cytotoxicity of anticancer platinum drugs: evidence that cis-diamminedich-loroplatinum (II) and cisdiammine (1,1-cyclobutane di-carboxylato platinum (II) differ only in the kinetics of their interation with DNA. Cancer Res. 1986,46:1972 7. Zwelling LA, et al. DNA-protein and DNA interstrand cross-linking by cis-and-trans-platinum(II) di-amminedichloride in I_iz, mouse leukemia cells and rela-tion to cytotoxicity. Cancer Res. 1979,39:365
计量
- 文章访问数: 1034
- HTML全文浏览量: 27
- PDF下载量: 243