胃癌c－Ha－ras基因突变分析方法的建立及临床应用研究

基金项目: “863”基金

Establishment and Clinical Application Study of C-Ha-ras Gene Mutation Analysis Methods in Gastric Cancer

摘要: 本文应用多聚酶DNA链延伸反应产物限制性酶切片段多态性分析（PCR-RFLP）和单链构象多态性分析（PCR-SSCP）两种方法同时检测了20例胃癌中c-Ha-ras基因突变。本文显示PCR-RFLP和PCR-SSCP均是检c-Ha-ras基因突变的简便、快速和敏感的方法，适用于临床大批量肿瘤标本的检测。
- 胃癌 /
- c－Ha－ras突变 /
- PCR－RFLP /
- PCR－SSCP
Abstract: In this work，mutations of c-Ha-ras gene in 20 cases of primary gastric cancer were investigatedby using restriction fragments length polymorphism (RFLP) and single-strand conformation poly-morphism (SSCP)analysis of the polymerase chain reaction product. We found that these two meth-ods were highly simple，rapid and sensitive assay system for detecting c-Ha-ras gene mutations oflarge number of tumor samples and PCR-SSCP could also detect c-Ha-ras point mutations in severalcodons at same time.
- gastric carcinoma /
- c-Ha-ras mutation /
- PCR-RFLP /
- PCR-SSCP

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