Abstract: Total 54 cases of human breast neoplasms were examed by using of Flow Cytometry (FCM)to get such parameters as DNA Index(DI),Proliferating Index(PI),S-phase fraction,of which the A_gNOR and 5-14 years follow-up in 34 casesof breast carcinoma were carried out.The results show that DI, PI and S-phase fraction are indicaters of distinguashing malignant and benign neoplasms of the breast;A_gNOR.DI.PI and S-phase fraction are related to the survival rate of breast carcinoma, which can be used as prognostic factors.