食管鳞状细胞癌DNA倍体异质性及其与患者预后关系的研究
Prognostic Significance And Heterogeneity In DNA PloidyOf Esophageal Squamous Cell Carcinoma
-
摘要: 本研究采用多点取材的方法，应用流式细胞术对80例中晚期食管鳞状细胞癌的瘤体内DNA倍体异质性进行了研究。DNA异倍体的检出率为88.8%（71/80），DNA指数范围为0.77～1.74。80例标本中，38例瘤体内表现为DNA倍体异质现象（47.50%）。DNA倍体异质性肿瘤患者的5年生存率（34.2%）极显著地低于非异质性肿瘤患者（64.3%）（P<0.025）。文中对瘤体内DNA倍体异质性的可能意义和发生机制进行了讨论。Abstract: Intratumoral heterogeneity in DNA ploidy was investigated in 80 cases of squamous cell carcinoma of the esophagus.Nuclear DNA content was determined formultiple samples obtained from the same tumor,using a flow cytometric(FCM)technique. The incidence of DNA aneuploidy was 88. 8% in this series, and DNA indicesranged from 0. 77-1. 74 of 80 cases,38 (47. 5 %)showed intratumoral heterogeneityin DNA ploidy. The 5-year-survival rate was significantly lower in the patients withtumors of heterogeneity in DNA ploidy(THDP)than those with non-THDP(P0. 025).Significance and mechanisms responsible for intratumoral heterogeneity inDNA ploidy are discussed from the aspect of tumor progression.
-
Key words:
- Esophageal carcinoma /
- DNA content /
- Ploidy /
- Heterogeneity /
- Prognosis
-
-
1. Nowell PC. Mechanisms of tumor progression. Cancer Res, 1986. 46:2203. 2. Nicolson GL. Tumor cell intability, diresification, and progression to the metastatic phenotype From onco-gene to oncofetel expression. Cancer Res, 1987, 47:1473. 3. Ljungberg B. Stenling R. Roos G. DNA content in renal carcinotna with reference to tumor heterogeneity. Can-cer. 1985. 56:503. 4. Quirke P, Dyson JED, Dixon NF, et al. Heterogeneity of colorectal adenocarcinoma evaluated by flow cytom-etry end histopathology. Br J Cancer, 1985. 51:99. 5. Sasaki K. Hashimoto T. Kawachino K. et al. Intratu-moral regional diffrences in DNA ploidy of gastroin-testinal cancer. Cancer. 1988. 62:2569. 6. Carey FA, Lamb CC. Intratumoral heterogeneity of DNA content in lung cancer. Cancer, 1990, 65:2266. 7. Sasaki K. Murakami T. Nakamura M. et al. Intratu-moral heterogeneity in DNA ploidy of esophageal squa-mous cell carcinomas. Cancer. 1991，68:2403. 8. Ruol A. Segalin A, Panozzo M, et al. Flow cytometric DNA analysis of squamous cell carcinoma of the esoph-agus. Cancer. 1990, 65:1185. 9. Jin-Ming Y, LI-Hua, Guo-Qian. et al. Flow cytometric analysis DNA content in esophageal carcinoma: Corre-lation with histologic and clinial features. Cancer,1989, 64:80. 10 Rognum TO, Lund E, Meling GI. et al. Near diploid large bowel carcinomas have better five-year survival than aneuploid ones. Cancer. 1991，68:1077. 11 Wainscoat JS.Fey MF. Assessment of clonality in hu-man tumors. Cancer Res. 1990. 50:1335. 12 Nowell PC. Cytogeneitics of tumor progression. Can-cer, 1990,65:2172.
计量
- 文章访问数: 950
- HTML全文浏览量: 31
- PDF下载量: 269
下载:
