Abstract: Eighty colo-rectal cancer tissues were assayed by using E₂-HRP and P_g-HRP affinity histochemical method for contents of ER and P_gR.The positiue rates of ER and P_gR in Large intestine cancer were 45. 0% and 36. 3% respectively. There were no correlation between ER or P_gR positivity and the sex of patient or location of tumor, metastasis of lymph nodes, type of tumor. The higher patients age and the better differentiation of cancer was the higher of the level of the ER and P_gR contents would be.It is indicated that large intestine cancer may be an estrogen dependent tumor and endocrinotherapy might be of help.