癌旁组织增殖特性的流式细胞测量术分析
Flow cytometric Study of Ploliferous Characteristics in Para-cancer Tissue
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摘要: 作者用流式细胞测量术对304例常见恶性肿瘤患者癌旁组织增殖特性进行了研究。结果表明，癌旁组织S期细胞比率(SPF)和超二倍体细胞比率(>2C%)均显著高于正常对照组织(P<0.01)，与癌中心灶十分接近。癌旁组织的DNA倍体类型不同，其>2C%明显不同，异倍体者显著高于二倍体(P<0.05)；癌旁组织倍体类型对其SPF影响不明显。癌中心灶DNA倍体类型对癌旁组织的>2C%和SPF未见明显影响。随肿瘤体积增大，癌旁组织的SPF有逐渐减低的倾向，其中肝癌旁组织表现最明显(R=-0.968，R>R0.01)；随肿瘤增大，癌旁组织超四倍体细胞比率(>4C%)呈递增的变化(R=0.84，R>R0.05)。Abstract: Proliferous Characteristics were measured on 304 samples of para-cancer tissue in diseases with common malignant tumor. The results showed that SPF (S-phase fraction) and 2C% (high diploidy percentage) of para-cancer tissue were higher than control (p0.01). SPF and 2C% of para-cancer tissue were approximate to cancer nests. Frequency of 2C% in para-cancer tissue was difference of itself ploidy types.20% of aneuploidy tumor was higher than that of diploidy tumor (p0. 05). The frequency of SPF in para-cancer tissue not affected by itself ploidy types. The frequency of2C% and SPF not affected by ploidy types of cancer nests. The frequency of2C% para-cancer tissue not observed.regular change, while that of SPF was a tendency of dicrease with increment of volume in cancer. change of hepatocellular cancer was evidentest in all cancer (r=- 0. 968, rr 0. 01). Percentage of high ploidy cells increased with increment of volume in cancer (r=0. 84, rr 0. 05).
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Key words:
- Flow cytometry /
- Cell cycle /
- Solid Tumor
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