Abstract: In this study, we extracted Gastric Cancer Antigen (GCaAg) with 3MKCl, immunized mice and prepared specific tramsfer Factor (STF). WithM Migration inhibitory test (MIT ). i, e. mouse lymphocytes sensitized by GCaAg adding only GCaAg and adding both STF and GCaAg were done. During the Test, the testing group's Mrp migration area is obvlously smaller than that of contrasting group's area. (P＜0.001 and P＜0.01). The migration Index (MI) are all positive under 0.8. During MTT Colorimetry, the Values of OD adding GCaAg groups and adding STF groups were obviously larger than that of contrasting groups (P＜0.05and P＜0.001 ). The lymphocyte transformation lndex of STF groups are the highest (1.73). The both tests suggest that STF did have the effect of ativating lympho cyte and show that Ag dependent specific activation. From MIT test, show that STF effect has no species limit, and this provides the basis for treat human tumor with STF which came from animals.