GSTM1和T1基因多态性与AFT暴露和肝癌危险关系的研究
Polymorphisms of GSTT1 and M1 Genotypes and Their Effects on Elevated Aflatoxin Exposure and Increased Risk of Hepatocellular Carcinoma
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摘要: 应用PCR法检测肝癌病例及对照GSTT1和M1空白基因并结合测定其黄曲霉毒素血清白蛋白加合物水平, 结果表明:GSTT1和GSTM1空白基因在肝癌病例和对照中分布不均衡, 同两种基因均为非空白型者比较, 仅GSTT1为空白基因型者患肝癌危险增加2.43倍(OR＝2.34，95%CI 1.01～5.46), 而两种基因均为空白基因者则患肝癌危险增加了2.43倍(OR＝3.43, 95%CI 1.50～7.91), 且血清中AFT－HSA加合物水平明显增高(P＜0.05); AFT－HSA与肝癌危险度间生物学梯度关系具有统计学显著性意义。 提示GSTT1和M1基因与黄曲霉毒素白蛋白加合物可能是筛检肝癌高危个体具有应用价值的一类生物标志物。Abstract: Genotypes of GSTT1 and M1 were determined by PCR, and the level of aflatoxin B1-albumin adduots in serum samples was examined by ELISA in HCC patients and healthy controls recruited in three case-control studies, conducted in Hebei, Jiangsu and Guangxi, China. It was found that compared with those with non-null genotypes of both GSTT1 and M1, the odds ratios of developing HCC for those with null genotype of GSTT1 and null genotypes of both GSTT1 and M1 were 2.34(95%CI 1.01～5.46) and 3.43(95%CI 1.50～7.91), respectivly. It was also found that Astatistlcally significant biological gradient between serum level of AFBalbumin adducts and risk of HCC. These findings imply That Genotypes of GSTT1and M1 and AFB-HSA may be employed in screening the persons at risk of HCC.
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