胃癌活检组织C-erbB-2及K-Sam基因扩增研究

Study on C-erbB-2 and K-sam Gene Amplification of Bioptic Tissues in Gastric Cancer

摘要: 应用PCR技术结合Southernblot分子杂交检测87例胃癌活检组织CerbB－2及K－sam扩增。结果显示：87例中有18例（20．69％）C－erbB－2扩增阳性，其中进展期胃癌、乳头状腺癌及分化良好型胃癌阳性率分别显著高于早期胃癌、其它组织类型胃癌及分化不良型胃癌（P<0.05）；伴淋巴结转移及肝转移胃癌阳性率显著高于不伴淋巴结及肝转移者（P<0．01和P<0．05）。5例胃癌K－sam扩增阳性，仅见于分化不良型胃癌（P<0．05）。该结果可作为术前胃癌估计预后的一项指标。

Abstract: The C-erbB-2 and K-sam gene amplitication of bioptic tissues m gastnc cancer of eighty-seven patients were examined by PCR and Southern blot hybridization.Of the eighty-seven cases,eighteen were positive for C-erbB-2 gene amplification.The positive rate was significantly higher in advanced cancers (P<0.05),papillary adenocarcinoma (P<0.05) and differenriated cancers (P<0.05).The C-erbB-2 gene amplification was found more often in cancers with lymph node and liver metastasis(P<0.01 and P < 0.05 respectively).K-sam gene amplification in five cases was only found in poorly differentiated cancers (P<0.05).The method can be probably used to be a factor of prognosis for gastric cancer before surgery.