非小细胞肺癌组织P<sup>53</sup>基因突变的研究

非小细胞肺癌组织P⁵³基因突变的研究

摘要: 本文旨在研究非小细胞肺癌患者发生抑癌基因P⁵³基因的突变量的状况，经采用PCR-SSCP法分析63例非小细胞肺癌和5例正常胎儿肺组织在外显予5、6、7和8中发生的点突变，NSCLC总突变率为49．2%（31／63）。5例正常胎肺组织未出现P⁵³基因突变，63例NSCLC患者中，37例磷癌，16例为腺癌和10例鳞除混合癌，其突变率分别为48．6%，50%和50．0%，与肿瘤组织类型无关。Ⅰ期19例，Ⅱ期22例和Ⅲ例22例，其突变率分别为47.5%，45．4%和54．5%，Ⅲ期突变率稍高，但无统计学意义。本结果证明肺癌组织存在P⁵³基因点突变可能为肺癌发生的关键基因之一。

Abstract: The present report was to investigate into P⁵³ NSCLC.Point mutation in exon and were gene mutation in patients with examined by polymerise chain reaction and single-strand conformation polymorsphism analysis in tumor tissues and embryonic tissue of the lung.Somatic Ps' mutations were detected in tumors Ps' gene alteration and According to TNM Stages，here was no difference NSCLC staging，such as for stage examined stage B and for stage Q .No Ps' mutation was embryonic tissues of the lung.Ps' mutations were detected in of between.