Significance of agnors in gastroscope-brushing cytology
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摘要: 本文对照组织学应用银染技术对49例病人胃镜刷检细胞进行了核仁组成区相关嗜银蛋白定量研究。其中腺癌16例、鳞癌21例、非典型增生5例、慢性炎症7例。结果表明，随鳞状上皮病变级别增高，AgNoRs均数也逐级增高；腺癌细胞AgNoRs均数明显高于正常腺上皮细胞（P<0．05）；正常细胞与癌细胞AgNoRs数目、形态、大小及分布都有显著差异。应用AgNoRs染色观察，使细胞学与组织学的诊断符合率由原来的75．51%提高到83．67%。表明AgNoRs在胃镜刷检细胞学中，同样是反映细胞增殖水平的敏感指标，尤其在大规模的细胞学普查中，可能有较大的实用价值。Abstract: Smers of gastroscope-brushing Cytology in 49 patients were examined with Ar-gyrophilie nucleolar organizer regionstain technique, the final diagnoses were based on the pareffin-embowered tissue sections, among which 16 adenocarcinomas, 21 squamous cell carcinomas 5 atypical dysplasias and 7 chronic inflammations. Rsscults showed that the mean number of AgNoR_s increased in proportion to the severity of lesion of the squamous cells; and more AgNoR_s in adenocareinoma cell than that in normal glandular epithelium cell were observed (P<0.05); Agnors in cancer cell are different from that in normal cells in amount, shage, size and disstri-bution within nucleus. The Conformance rate of cytology to histology increased from 75.51% to 83.67% by means of AgNoR_s determination. It suggested that Ag-NoR_s is a comparatively senstive index to reflect proliferative activity of cells in gas-troscope-brushing cytology. and may apply more practical utilization to general sur-vey of esophgeal and gastric diseases.
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Key words:
- nucleolar organizer region /
- cytology /
- adenocarcinoma /
- squamous cell carcino-ma /
- atypical dysplasia
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