人神经母细胞瘤SK-H-SN细胞株的多药耐药性及逆转研究

详细信息

Multidrug Resistance and Reversion study on Human Neuroblastoma SK-H-SN Cell Line

1. Department of Surgery, Pediatric Hospital;2. Shang Hai Medical University, 200032

摘要: 本文对人神经母细胞瘤SK－H－SN细胞株的多药耐药性及其逆转进行实验，LSAB免疫组织化学定位显示整个SK－H－SN细胞膜表面以及细胞质内都有较强的耐药性P-170糖蛋白；MTT细胞毒性分析法证实，分别合用维拉帕米和双嘧啶胺醇前后，SK－H－SN细胞株细胞对抗肿瘤药物阿霉素、长春新碱的敏感性均有显著性差异（P<0．01）。表明维拉帕米和双嘧啶胺醇对人神经母细胞瘤SK－H－SN细胞株的耐药性有逆转作用。
- 阿霉素 /
- 长春新碱 /
- 耐药性 /
- 维拉帕米 /
- 双嘧啶胺醇
Abstract: Experiment was done on human neuroblastoma SK-H-SN cell line about its multidrug resistance and reversion. with ABC immunohistoehemical localization, both the cytoplasma and cell membrane showed abundant P-glycoprotein staining,MTT cytotoxic assay revealed that the sensitivities of SK-H-SN cells to doxoruieine or vincristine were significantly different according to the presence or absence of ve-rapamil or dipyridamole(P<0.0 1), which indicate that the drug resistance of SK-H-SN cells can be reversed by verapamil or dipyridamole.
- Doxorubicine /
- Vincristine /
- Drug resistance /
- Verapamil /
- Dipyridamole

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