RB、P53抑癌基因蛋白在甲状腺肿瘤中的表达
Expression of retinoblastoma and P53 tumour suppressor gene protein in human thyroid tumours
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摘要: ABC免疫组化法检测77例甲状腺肿瘤和非瘤病变中RB与P53抑癌基因蛋白的表达。所有标本中都有RB蛋白表达,表明RB基因失活在甲状腺肿瘤中不起重要作用。甲状腺非瘤病变与腺瘤中P53全部阴性,甲状腺乳头状癌、滤泡状癌中P53阳性率低(8.3％与15.8％),阳性细胞数也少(<10％),P53主要在甲状腺未分化癌中高表达(66.7％)且与乳头状癌,滤泡状癌有显著性差异,提示P53蛋白异常在甲状腺癌由高分化向未分化发展过程中起关键性作用。Abstract: We have examined retinoblastoma (RB) and P53protein by immunohistochemistry inseventy-seven formalin fixed, paraffin wax embedded thyroid lessions.Thyroid lessionsstudied included 10 non-neoplastic thyroid tissues, 14 thyroid adenomas, 24 papillarycarcinomas, 19 follicular carcinomas, 9 undifferentiated carcinomas and 1 medullary car-cinoma.The RB protein is not loss in any cases, indicating that inactivatlon of the RBgene is unlikely to play a important role in the pathogenesis of thyroid tumours.Six of 9(66.7%) undifferentiated carcinonlas, 3 of 19(15.8%) follicular carcinomas, 2 of 24 (8.3%) papillary carcinomas show P53protein nuclear staining.In all of P53positive cases, 4of 6 undifferentiated carclnomas showed more positive cells than 5 well-differentiatedcarcinomas in which less than 10 percent of cells had increased P53protein levels.Our results suggest that P53protein abnormalities play a crucial role in the progression of well-differentiated to undifferentiated thyroid carcinomas.
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Key words:
- P53protein /
- RB protein /
- thyroid neoplasmas /
- immunohistochemistory
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