亚硒酸钠对人早幼粒白血病细胞的RB蛋白磷酸化的影响

刘新光; 于树玉

亚硒酸钠对人早幼粒白血病细胞的RB蛋白磷酸化的影响

刘新光¹,
于树玉²

1. 广东医学院医用生化研究所, 湛江 524023;2. 中国医学科学院肿瘤研究所生化室

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- 刊出日期: 1998-08-04

Effect of sodium selenite on phosphorylation of retinoblastoma protein of human promyelocytic leukemia cells (HL-60)

Liu Xinguang¹,

Biochemistry institute, Guangdong Medical College, Zhanjiang 524023

摘要

摘要: 以人早幼粒白血病细胞(HL－60)为实验对象,利用Western－blotting方法和流式细胞仪分析,研究亚硒酸钠对HL－60细胞的RB蛋白产物磷酸化的影响和探讨其抗癌机制。Western－blotting结果显示:与对照组相比,在第四天5.8μmol/LNa2SeO3可使磷酸化Rll蛋白减少49％,去磷酸化RB蛋白仅轻度增加,但在加硒组中去磷酸化RB蛋白占RB蛋白总量的71.1％。与此同时,细胞周期的结果表明:亚硒酸钠对HL－60细胞的G2＋M和S期有一定的阻滞。该实验在蛋白分子水平上研究了亚硒酸钠抑制HL－60细胞增殖的作用机制。
- 亚硒酸钠 /
- HL－60细胞 /
- RB蛋白磷酸化 /
- Western－blotting /
- 细胞周期
Abstract: Human promyelocytic leukemia cells (HL-60) was used to study the effect of sodiumselenite on phosphorylation of retinoblastoma (RB) protein and its anticancer mecha-nism.Method of western--blotting and flow cytometric analysis were undertaken.Theresult of western-blotting showed that 4 days after treatment of 5.8μmol/L.Na2SeO3,the amounts of the phosphorylated RB protein decreased some 49%that of controlgroup,while the hypophosphorylated RB protein increased a little.However, in this selenium group the phosphorylated RB protein was 71.1% in total RB protein amounts.In the same time, the result of cell cycle revealed that sodium selenite could slightly arrest HL-60 cells at G2+M and S phase.The experiments studied the mechanism of sodium selenite inhibited HL-60 cells proliferation at protein molecules level.
- Sodium selenite /
- Human promyelocytic leukemia cell line (HL-60) /
- Retinoblastoma protein phosphorylation /
- western--blotting /
- cell cycle

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参考文献(12)

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[3]	Brooks SC, Kazmer S, Levin AA,et al. Myeloid differen-tiation and retinoblastoma phosphorylation changes in HI_-60 cells induced by retinoic acid receptor-and retinoid X re-ceptor-selective retinoic acid analogs. Blood,1996,87:227
[4]	Sellers WR, Kaelin WG. RB as a modulator of transcrip-tion. Biochim Biophys Acta, 1996,]288: MI
[5]	Chen PL,Scully P, Shew JY, et al. Phosphorylation ofthe retinoblastoma gene product is modulated during thecell cycle and cellular differentiation. Cel1,1989,58:1193
[6]	Mihara K, Cao XR, Yen A, et al. Cell cycle-dependentregulation of phosphorylation of the human retinoblastomagene product. Science,1989,246:1300
[7]	Lowry OH,Rosebrough NJ,Farr AI,et al. Protein measurement with the Folin phenol reagent. J Biol C hem,1951,193:265
[8]	Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T,. Natrue, 1977,227:680
[9]	Jing YK, Hashimoto S, Nakajo S, et al. Topoisomerase in-hibitors potentiate the effect of retinoic acid on cell growth inhibition and induction of differentiation of leukemia HI 60 cells. Leukemia Res,1991, 18:299
[10]	Chen PL,Riley DJ,Lee WH. The retinoblastoma protein as a fundamental mediator of growth and differentiation signals. Crit Rev Fukaryot Gene. Fxpr,1995。5:79
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亚硒酸钠对人早幼粒白血病细胞的RB蛋白磷酸化的影响

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Effect of sodium selenite on phosphorylation of retinoblastoma protein of human promyelocytic leukemia cells (HL-60)

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亚硒酸钠对人早幼粒白血病细胞的RB蛋白磷酸化的影响

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Effect of sodium selenite on phosphorylation of retinoblastoma protein of human promyelocytic leukemia cells (HL-60)

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出版历程

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