Abstract: Hepatoma was induced in rats by administration of diethylnitrosamine(DEN).The normal rats and hepatoma rats were treated controls were not treated w.ith any immune stimulants(IS). The peritoneal macrophages, lung and spleen macrophages, monocytes were isolated from normal and hepatoma rats (at 8th, 12th and 16th week) and cocultured with human SMMC-7721 hepatoma cell,the activity of IL-1 in media were measured by 3TdR incorporation of, thymocytes isolated from mice; the peritoneal macrophages, lung and spleen macrophages, monocytes were isolated from control hepatoma rats(at 18th week)and treated with IS in vitro, and were then cocultured with human SMMC-7721 hepatoma cell,the activity of IL-1 in media were assayed. The results showed that both in normal and hepatoma rats, either in vivo or in vitro, IFNα-2b and BCG enhanced monocytes and macrophages to release IL-1, particully,in combination with IFNα-2b and BCG groups which increased the production of IL—1 by 0.09~1.04 times.The results suggested that the combined application of IS to patients suffering from hepatoma may be much better than the iS used alone.