Abstract: The induction of apoptosis in human ovarian carcinoma cell line AO10/17 was morphologically and biologically examined by 5-fluorouracil (5—Fu).It has shown that AO10/17Underwent apoptosis as characterized by morphologic changes with chromatin condensation and DNA fragmetation after treated by 5-Fu.A flow cytometric method formeasuring the percentage of apoptotic nuclei (hypodiploid DNA)after PI staining and colormetric method measure DNA fragmentation in the nucleatr extracts,both of them were higher in treated cells than is untreated cells.The date demonstrates effection of 5—Fu on AO10/17 is associated with apoptosis.