P16抑癌基因在卵巢肿瘤中的表达
Expression of Tumor Suppressor Gene P16 in Ovarian Neoplasm
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摘要: 目的:研究抑癌基因P16与卵巢癌的关系, 方法:用免疫组织化学及原位杂交方法检测T72例卵巢肿瘤组织中P16抑癌基因蛋白及其mRNA的表达情况。 结果:免疫组化显示:P16在卵巢癌、良性卵巢肿瘤、交界性肿瘤及正常卵巢组织中的检出率分别为:7.89％、60.00％、6.67％和83.30％, 各组与卵巢癌组相比有显著差异(P<0.01)。 原位杂交表明在上述不同组织中P16mRNA的阳性率分别为21.05％、70.00％、58.33％和75.00％, 各组与卵巢癌组相比有显著差异(P<0.05)。结论:P16在卵巢癌中的表达明显低于其它卵巢肿瘤及正常卵巢组织中, 表明P16作为一种重要的抑癌基因, 它的突变与缺失可能与卵巢癌的发生发展密切相关Abstract: Objective: To observe the relationship between tumor suppressor gene P16 expression and ovarian cancer. Methods: ABC Immunohistochemistry and In sttu hybridizationmethods were used to study the expression of P16 in 72cases of ovarian tumors and normal ovarian tissue. Results: Immunohistochemical results show that the positive rate ofP16 protein in ovarian cancer, benign tumor, borderline tumor and nomal ovarian tissue is 7, 89%, 60.00%. 66.67% and 83.30% respectively. While the oisitive rate of P16mRNA from in situ hybridization in the tissues mentioned above is 21.50%, 70.00%,58.33% and 75.00% reSPectively. The plsitive rate of P16 protein and mRNA in ovarian cancer is significant lower than that in other tumors and normal ovarian tissue. Conclusions: P16is an important tumor suppressor gene, the mutation and deletion of P16 arewell related with the occurence and development of ovarian cancer.
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Key words:
- Ovarian neoplasm /
- P16 /
- Tumor suppressor gene /
- Imnmunohistoche-mistry /
- In situ Hybridization
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[1] Kamb A, Nellere A, et al. A cell cycle regulator potentially involved in gensis of many tumor types. Science, 1994, 12(264):436～440. [2] Laura Bonetta. Open question on P16. Nature, 1994, 21(370):180～182. [3] Serrano M, et al. A new regulatory motif in cell cycle con-trol cansing specific inhibition of cyclin D/CDK4. Nature, 1993, 15(366):704～707. [4] 李楠, P16基因与肿瘤, 国外医学肿瘤学分册, 1995, 22(5):257～259. [5] Marx. A Challenge to P16 ene as a major tumor suppres-sor. Science, 1994, 24(264):1846～1847. [6] Osborne R. J, Leech. Polymerase chain reaction allelotyp-ing of human ovarian. Cancer, 1994, 69:429～438. [7] David CS, Lisa V. Characterization of chromosome 9 in hu-man ovarian neoplasm identifies frequent geneti imbalance on 9q and rare alterations involving 9q, including CDKN2, Cancer Res, 1995, 15(55): 2150～2157. [8] James Eberwine, et al. The Use of In Situ Transcription in the Study of Gene Expression. In Chesselet M. F:In situ Hybridization. Boston, 1990:141.
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