人胃癌SGC-7901细胞胞浆胞核性激素受体测定

The sex hormone receptors in cytosol and nuclear extract fractions of human gastric cancer SGC-7901 cell line

摘要: 作者采用葡聚糖包被活性碳饱和分析法(DCC法)分别测定了培养的人胃低分化腺癌SGC-7901细胞的胞浆及胞核KCL抽提液中的雌激素受体(ER)、孕激素受体(PR)和雄激素受体(AR)的含量。结果证明胃癌SGC-7901细胞的胞浆及胞核抽提液中ER含量为30.2和35.7fmol/mg蛋白，而PR的含量为20.3和22.7fmol/ng蛋白, 但AR均为阴性(<10fmol/mg蛋白), 这说明SGC-7901细胞为ER及PR阳性细胞, 其ER和PR在胞浆、胞核中均有分布。提示胃癌可能为性激素依赖性肿瘤, 有内分泌治疗的可能。
- 胃癌 /
- 雌激素受体 /
- 孕激素受体
Abstract: We determined the levels of estrogen(ER), progesterone(PR) and androgen recepter(AR) with dextrancoated charcoal method(DCC method) in both the cytosol andnuclear KCI extractable fractions separated from cultured SEC-7901 cells, a humanlower differentiated gastric adenocarcinoma cell line. The result showed that, in cytosoland nuclear extract of SGC-7901 cells the level of ER were 30.2 and 35.7 fmol/mgprotein, the level of PR were 20.3 and 22.7 fmol/mg protein, but the AR were negative(10fmol/mg protein) individually. This result indicated that SGC-7901 cell is ERand PR positive, the ER and PR existed in cytosol and nuclear extract of gstric carcinomacells. Soogastric cancer is possibly sex hormone dependent and responding to endocrinetherapy.
- Gastric cancer /
- estrogen receptor /
- Progesterone receptor

[1]	Kiang DT. The presence of steroid recepters in "nontar-get" tissues and it's significance. Am J Cli Pathol, 1993, 99:120.
[2]	Tokunaga A, Nish K, Matsuhura N, et al. Estrogen and proesterone receptors in gastric cancer. Cancer, 1986, 57:1376.
[3]	Wu CW, Chi CW, Chang TJ, et al, Sex hormone receptors in gastric cancer. Cancer, 1990, 65:1396.
[4]	石晓毅, 沈明, 江萍等. 胃癌109例雌激素受体与孕激素受体的检测研究. 中国肿瘤临床, 1995, 65:1396.
[5]	李玉军, 孙显露, 孙惠安, 等. 胃癌雌激素受体的临床生物学意义. 肿瘤防治研究, 1995, 22:363.
[6]	Lin CH, Investigation of SGC-7901 cell line established from human gastric carcinoma cell. Chinese Med J, 1984, 97:831.
[7]	Virolainen E, Vanharanta R, Carey TE. Steroid hormone receptors in human squamous carcinoma cell line. Int. J Cancer, 1984, 33:19.
[8]	刘占举, 徐志林, 刘学勤, 等. DCC法检测食管鳞癌中雌孕激素胞浆胞核受体. 中国临床肿瘤, 1995, 11:53.
[9]	刘以训, 雌孕激素受体测定. 生殖与避孕, 1981，1:53.
[10]	谭瑞军, 谷志远, 汪鸿志, 等. 雌激素及其受体抑制剂在体外对人胃癌细胞增殖的影响. 中华肿瘤杂志, 1995, 17:93.
[11]	Harrison JD, Morris DL, Ellis IO, et al. The effect of tamoxifen and estrogen receptor status on survival in gas-tric carcioma. Cancer, 1989, 64:1007.