幽门螺杆菌感染与胃腺上皮细胞增生关系的研究
The association between belicobacter pylori infection and epithelial cell hyperplas of gastric gland
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摘要: 目的:研究幽门螺杆菌(HP)与胃腺上皮细胞增生的关系, 方法:经PCR检测和组织病理学观察138例胃粘膜活检标本, 采用SP免疫组化染色观察PCNA表达和Feulgen二染色测定细胞核DNA含量。 结果:细胞核DNA含量在慢性浅表性胃炎(CSG)和慢性萎缩性胃炎(CAG)中, 有HP感染与无HP感染比较, 二倍体和近二倍体细胞减少, 增殖倍体增加。 PCNA表达在CSG和CAG中, 有HP感染与无HP感染比较阳性细胞数和均值增加。 结论:HP感染与胃腺上皮细胞的增生程度有密切关系。Abstract: To study the association between helicobacter pylon(HP) infection and epithelialcell hyperplasia of gastric gland. 138 cases of gastric mucosa from the polymerase chainreaction(PCR) technique and observations of histopathology were performed with PCNAexpression of S-P immuncocy to chemical method and DNA content exanines of Feulgen staining. RESULTS: HP infection and non-HP infection compared with diploid andnear diploid cells reduces and proliferating diploid cell increased, in the chronic superficialgastritis(CSG) and the chronic atrophic gastritis(CAG) on the study of the DNA content of cell nucleus. HP infection and non-HP infection compared with increased of positive cells, in the expression of PCNA with S-P immunohistochemical staining CONCLUSION: The degree of the epithelial cell hyperplas of gastric gland was very correlative to HP infection.
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[1] Nomura A, Stemmermann GN, Chyou PH, et al. Heli-cobacter pyfori inlection and gastric carcinoma among japanese American in Hawaii. N Engl J Med, 1991, 325:1132. [2] Parsonner J, Friedmam GD, Vandersteen DP, et al. Helicobacter pylori infection and the risk of gastric careinoma. N Engl J Med, 1991, 325:1127. [3] Graanen Me, Dekker W, Ferwaerda J, et al. Intestinal metoplasia and Helicobacter pylori:an endoscopie bioptie study of the gastric antrum. Gut, 1992, 33:16. [4] Forman D, Neweel DG, Fullerton F, et al. Association between infection with Helieobacter pylori and risk of gastric cancer:evidence from a prospective investigation. BMJ, 1991, 302:1302. [5] 王仰坤, 马乃绪, 董荣春, 等. 慢性萎缩性胃炎的组织病理学分型及其意义的研究. 解放军医学杂志, 1993, 18(4):288. [6] 王仰坤, 纪小龙, 马乃绪, 等. 萎缩性胃炎中P53和PCNA的表达, 新消耗病学杂志, 1997, 5(4). [7] 王仰坤, 马乃绪. P21蛋白在萎缩性胃炎中的表达及DNA含量测定. 中华医学杂志, 1997. [8] Wang YK, Ji XL, Gu YG, et al. P53 and PCNA expression in glandular dilatation of gastric mucosa. China Natl J New Gastroenterol, 1996, 2(2):106.
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