食管癌术前放疗后PCNA蛋白表达的改变及预后
PCNA Prediction of Prognosis of Esophageal Squamous Cell Carinoma with in Preoperative Radiotherapy
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摘要: 目的 探讨放疗对食管癌PCNA蛋白表达的影响，指导临床选择有效治疗方案。方法在我院接受术前放疗及根治性食管癌切除术的91 例食管癌患者，前后两野垂直照射，肿瘤剂量30～40Gy15 ～20 次3 ～4 周。用免疫组化SP方法检测。结果 食管鳞癌呈100 % PCNA 蛋白表达，放疗后高表达率71-4% 高于放疗前的57-1 %, P= 0-04 ;疗后PCNA 高表达者较低表达者预后明显差，P= 0.0001 ;比较同一病人放疗前、后PCNA蛋白表达，显示放疗后下降者、升高者和不变者的预后均有显着性差别，P= 0.00001.结论 ①疗后PCNA蛋白表达升高，可能存在肿瘤细胞加速再增殖②PCNA高表达者预后很差；而放疗后与疗前相比，下降者生存最好，有可能成为评价预后指标。Abstract: Objective To study changes PCNA in radiotherapy of esophageal carcinoma and analyse the validity of PCNA expression on prognosis. Methods From Nov.1979 to Dec.1996,91 patients were treated by preoperative radiotherapy plus radical esophagectomy in our hospital.All were confirm as squamous cell carcinoma.Two vertical irradiation fields to deliver a dose of 30～40GY/15～20F/3～4W and PCNA with immunohistochemical staining. Results The overexpression rates wree 71 4% and 57 1% for the post and pre radiotherapy, P =0.04.The survival rate of overexpression were lower than those lowexpression in post radiation, P =0.0001.The various changes of PCNA between pre and post radiation,show different survival rates, P =0.00001.The survival time differed with different reduction of PCNA indexes. Conclusion ① PCNA protein expression rise after pre operative radiotherapy of esophageal carcinoma,may signify the repopulation of tumor cells.② The survival periods were poor for patients with overexpression and the prognosis was good for patients who show a conspicuous reduction of PCNA index after radiotherapy.
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Key words:
- Esophageal carcinoma /
- Radiotherapy /
- PCNA
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[1] 韩企夏，施学辉，范可成，吴永如.食管癌.见汤钊猷主编.现代肿瘤学.上海：上海医科大学出版社，1993，455 [2] 殷蔚伯.食管癌.见谷铣之，殷蔚伯，刘泰福主编.肿瘤放射治疗学.北京：北京医科大学中国协和医科大学联合出版社，1993，425 [3] Lapertosa G,Baracchini P,Fulcheri E.Asessment of PCNA expression in dysplastic intestinal type Barretts esophagus.Pathology,1994,86(2):174～179 [4] Kuwano H,Sumiyoshi K,Nozoe T,et al.The prognostic significant of the cytophoto metric DNA content and its relationship with argyrophilic nucleolar organizer (AgNOR) and proliferating cell nuclear antigen (PCNA) in esophageal cancer.Eur J Surg Oncol,1995,21:368～373 [5] 祝淑钗，殷蔚伯，万钧.增殖细胞核抗原蛋白表达与食管癌术前放疗预后的关系.中华放射肿瘤学杂志，1998，7(3)：166～169 [6] Micheal BN,Robert MB,Rogert BF,et al.Identify of the proliferating cell nuclear antigen and cyclin.Nature,1984,305(5):374～376 [7] Garcia RL, Coltrera MD, Gown Am. Analysis of proliferative grade using anti-PCNA/cyclin Monoclonal antibodies in fixed embedded tissues.Am J apthol,1989,134(4):733～739 [8] Bourhis. J,Dendale. R,Hill.C,et al.Potential doubling time and clinical outcome in head and neck squamous cell carcinoma treated with 70GY in 7 weeks.Int.J.Radiat.Oncol.Biol.Phys,1996,35(3):471～476 [9] 韩春，万钧，周道安，等.100例食管癌后程加速放疗的研究.中华放射肿瘤学杂志，1997，6(1)：16～18
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