食管癌中P16、PCNA基因蛋白的表达及意义
P16 and PCNA Expression and Their Significance in Human Esophageal Squamous Cell Carcinoma
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摘要: 目的：探讨P16、PCNA基因蛋白表达与食管鳞癌发生发展的关系。方法：应用S-P免疫组织化学方法对84例食管鳞癌及10例正常食管组织分别检测P16蛋白、PCNA蛋白表达。结果：84例食管鳞癌中P16蛋白表达率为54.8%(47/84)，P16表达与淋巴结转移、临床分期、术后生存期显著相关(P<0.05)；PCNA表达阳性率与食管癌组织分化程度显著性正相关(P<0.05)，淋巴结转移组显著高于未转移组，并与术后生存期互相关(P<0.01)，与临床分期无显著相关。P16与PCNA表达呈反相关系(P<0.01)。结论：表明P16在食管癌生长、转移中起重要作用，P16、PCNA可作为判断预后的重要指标。Abstract: Objective:To investigate the possible relationship betwween P16 PCNA expression and their significance in human esophageal squamous cell carcinoma Methods:84 resected esophageal carcinomas speciments was examined for the expression of P16 PCNA proteins by using immunohistochemical S P method Results:The expression of P16 gene was 54 8%.The lower the differentiation of esophageal carcinoma,the lower the P16 positive rate ( P 0 01).The positive rate was significantly related to the ability of node metastasis TNM staging and postoperative ( P 0 05).The PCNA positive cell rates of esophageal cancer were 12 9%、26 9%、and 47 5% in well、moderately and poorly differentiated carcinoma respectively and showed a significant negative correlation with survival the differentiation degree of the tumor and the ability of node metastasis( P 0 001). Conclusion :the results suggest expression of P16 in esophageal carcinoma play an important role in suppression of invasive growth and metastasis P16 and PCNA may serve as a marker of predicting metastasis and prognosis of esophageal carcinoma.
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