大肠癌中人乳头瘤病毒感染与p53关系的相关性研究
Relationship of Human Papillomavirus and P53 with Colorectal Carcinoma
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摘要: 目的 研究人乳头瘤病毒（HPV）与大肠癌的相关性以及p53的关系。 方法 应用聚合聚合酶链反应（PCR）和免疫组化技术分别检测对例大肠癌组织和15例正常结肠粘膜中的HPVDNA和p53蛋白。 结果 大肠癌中HPVDNA阳性9例（27．27％）,p53蛋白阳性18例（54．55％）；正常粘膜中二者均阴性9例HPVDNA阳性的大肠癌中p53蛋白阳性3例,另外6例阴性。 结论 大肠癌的发生与HPV感染有关（P＜0．05）,HPV感染与p53基因突变在大肠癌发生过程中可能单独起作用,HPV的致癌作用主要不是通过使p53基因失活这个途径。Abstract: Objective To assess the relationship of human papillomavirus(HPV)and p53 with colorectal carcinoma. Methods The 33 adenocarcinomas and 15 normal mucosal samples were selected for the current study using polymerase chain reaction(PCR) and immunohistochemical techniques. Results HPV DNA was detected in 9 of 33(27.27%)carcinomas;The expression of P53 protein was found in 18 of 33(54.55%)carcinomas;No HPV DNA and P53 expression were detected in all 15 normal colorectal specimens.Out of the 9 HPV DNA positive cases,only 3 cases with p53 protein expression were found. Conclusion HPV might be involved in the pathogenesis of colorectal carcinomas (P<0.05).HPV infection and p53 mutation may play a role independently in the carcinogenesis of colorectal cancer.
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