癌基因蛋白P21、P185、EGFR及LN、IV型胶原在骨肉瘤中的表达
Expression of p21 、p185 、EGFR 、LN and Type IV Collagen in Human Osteosarcoma Tissue
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摘要: 目的: 探讨癌基因蛋白p21、p185、EGFR及LN、IV型胶原在人骨肉瘤组织中表达的意义。方法: 应用免疫组化ABC法, 检测40例常规石蜡包埋的骨肉瘤组织中p21、p185和EGFR的表达, 同时还检测了LN、IV型胶原在骨肉瘤组织中的含量。结果: p21、p185和EGFR在骨肉瘤组织中的阳性表达率分别为26.3%(10/38), 65.8%(25/38), 50.0%(19/38)同时观察到LN和IV型胶原在骨肉瘤组织中往往是缺如的。结论: 骨肉瘤组织中存在着多种癌基因的异常, 它们与骨肉瘤的发生发展密切相关, 同时细胞外基质的破坏在骨肉瘤的侵袭与转移中起着重要的作用。Abstract: Objective: To evaluate the significance of p21, p185, EGFR protein expression and LN, type IV collagen in human osteosarcoma . Methods: p21, p185, EGFR protein expression and LN, type IV collagen were examined in 40 cases human osteosarcoma tissue with immunohistochemical ABC methods. Results: Accumulation of p21 protein was found in 8 out of 38 cases osteosarcomas. The positive rates of p185 andEGFR were respectively 65.8 % and 50.0 %. It was observed that the majority of LN and type IV collagen expression in osteosarcoma was defective. Conclusion: The results of p21, p185 and EGFR high expression in human osteosarcoma suggest that they did have a correlation with osteosarcoma origin and development; The changes of LN and type IV collagen have significant effect on osteosarcoma invade and metastasis.
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