视黄酸对胃癌细胞的诱导分化及其相关酶活性的影响
Effects of Retinoic Acid on Gastric Cancer Cells' Differentiation and Relevant Enzmye Activity
-
摘要: 目的: 研究视黄酸对胃癌细胞的诱导分化及其相关酶活性的影响。方法: 10-6的全反式视黄酸(ATRA)处理胃癌细胞株BGC-823和MKN-45用流式细胞术检测细胞周期的分布, 以酶标仪测定相关分化标志酶的活性, 用3H-TdR掺入法测定DNA合成速度和MTT法检测细胞的生长。结果: ATRA使BGC-823细胞出现G0/G1期停滞, 分化标志酶LDH、ALP和β-G的活性下降，抑制率分别为22.4%, 32.94%和41.35%，使DNA合成速率下降41.43%，生长抑制率为61.03%；但不能改变MKN-45细胞的上述指标。结论: ATRA能诱导BGC-823细胞分化并抑制其恶性生长, 对MKN-45细胞则没有这种作用。Abstract: Objective: To explore effects of retinoic acid on gastric cancer cells' differentiation and relevant enzmye activity. Methods: Treated with 10-6 mol/L all-trans retinoic acid(ATRA), gastric cancer cells, BGC-823 and MKN-45, were measured by flow cytometry for cell cycle distribution, by microplate reader for enzyme activity, by 3H-TdR incorporation method for DNA synthetic speed and by MTT method for cell growth rate. Results: After treated with ATRA, BGC-823 was lead to stay in G0/G1 phase, while LDH, ALP and β-G activity was suppressed, the inhibition rates were 22.40 %, 32.94 % and 41.35 %, respectively. DNA synthetic speed was decreased by 41.43 % and growth inhibition rate was 61.03 %. Nevertheless, MKN-45 can't be changed as BGC-823. Conclusion: BGC-823, but not MKN-45, can be induced differentiation and cell growth can be suppressed by ATRA.
-
Key words:
- Retinoic acid /
- Gastric cancer cells /
- Differentiation /
- Cell cycle /
- Enzyme activity
-
-
[1] Gudas LJ, etal. Cellular biology and biochemistry of the retinoids. In “The Retinoids”, 2nd ed., 1994, p443-520, Raven Prsdd ,New York; [2] 刘卫, 房殿春.维甲类化合物诱导肿瘤细胞分化研究进展.国外医学内科学分册, 1996 ,23: 160-163; [3] Kim YS .etal.Effects of sodium buti rate, dimethy sulfoxide and retinoic acid or glycolipids on human renal adenocarcinoma cells. Cancer Res, 1984, 44 :1648-1652; [4] 孙月霞, 等.维甲酸对人胃癌细胞表型的逆转作用.肿瘤, 1994, 14: 85-87; [5] M .Machlas, etal.The Determination of Lactic Dehydrogenase with a Tetrazolium Salt.Anal.Biochem, 1960, 1: 317-326; [6] Gracia-Rozas C, etal. Alkaline Phosphatase Activity as a Memberame Marker for Activated B cells. J Immunol, 1982, 129(1): 52-53; [7] Fichman WH, etal. Human Serum β-Glucuronidase; Its Measurement and Some od Its Properties. Clin Chim Acta, 1967, 15(3): 435-447; [8] 吴乔, 等.ATRA 受体转录水平的改变与癌细胞生长的关系.厦门大学学报(自然科学版), 1997, 36: 787-794; [9] 糜漫天, 等.ATRA 诱导细胞分化分子机制的研究进展.国外医学分子生物学分册, 1998, 20: 17-22; [10] Mehta K, etal. Activation of retinoid receptors RARαand RXRα induces differentiation and apoptosis, respectively, in HL60 cells.Cell Growth Differ, 1996, 7: 179-186; [11] Shao Z-M, etal. p53 independent G0/G1 arrest and apoptosis induced by a novel retinoid in human breast cancer cells. Oncogene, 199511: 493-504; [12] 汤钊猷主编.现代肿瘤学.上海:上海医科大学出版社, 1993: 47-70; [13] Nowak G, etal.Hypoxi a and proliferation are primory responsible for induction of lactate dehydrogenase activity in cultured cells.J Toxi cology &Envi ronmental Health, 1996, 49: 439-452.
计量
- 文章访问数: 1025
- HTML全文浏览量: 24
- PDF下载量: 343
下载:
