视黄酸对胃癌细胞的诱导分化及其相关酶活性的影响
Effects of Retinoic Acid on Gastric Cancer Cells' Differentiation and Relevant Enzmye Activity
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摘要: 目的: 研究视黄酸对胃癌细胞的诱导分化及其相关酶活性的影响。方法: 10-6的全反式视黄酸(ATRA)处理胃癌细胞株BGC-823和MKN-45用流式细胞术检测细胞周期的分布, 以酶标仪测定相关分化标志酶的活性, 用3H-TdR掺入法测定DNA合成速度和MTT法检测细胞的生长。结果: ATRA使BGC-823细胞出现G0/G1期停滞, 分化标志酶LDH、ALP和β-G的活性下降，抑制率分别为22.4%, 32.94%和41.35%，使DNA合成速率下降41.43%，生长抑制率为61.03%；但不能改变MKN-45细胞的上述指标。结论: ATRA能诱导BGC-823细胞分化并抑制其恶性生长, 对MKN-45细胞则没有这种作用。Abstract: Objective: To explore effects of retinoic acid on gastric cancer cells' differentiation and relevant enzmye activity. Methods: Treated with 10-6 mol/L all-trans retinoic acid(ATRA), gastric cancer cells, BGC-823 and MKN-45, were measured by flow cytometry for cell cycle distribution, by microplate reader for enzyme activity, by 3H-TdR incorporation method for DNA synthetic speed and by MTT method for cell growth rate. Results: After treated with ATRA, BGC-823 was lead to stay in G0/G1 phase, while LDH, ALP and β-G activity was suppressed, the inhibition rates were 22.40 %, 32.94 % and 41.35 %, respectively. DNA synthetic speed was decreased by 41.43 % and growth inhibition rate was 61.03 %. Nevertheless, MKN-45 can't be changed as BGC-823. Conclusion: BGC-823, but not MKN-45, can be induced differentiation and cell growth can be suppressed by ATRA.
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Key words:
- Retinoic acid /
- Gastric cancer cells /
- Differentiation /
- Cell cycle /
- Enzyme activity
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