反义寡核苷酸对Daudi细胞α2,6唾液酸的下调作用研究
A Study of Antisense-Oligonucleotides in Down-Regulation of α2,6-Sialylation of Daudi Cells
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摘要: 目的 研究反义寡核苷酸对Burkitt's淋巴瘤Daudi细胞α2, 6 唾液酸转移酶 (ST6GalI)的活性和细胞表面α2, 6 唾液酸水平的下调作用。方法 以修饰后的ST6GalI的反义寡核苷酸处理Daudi细胞, 以RT PCR检测Daudi细胞ST6GalImRNA的表达, 荧光定量法测定ST6GalI的活性及以流式细胞仪检测细胞表面α2, 6 唾液酸的含量。结果 和对照组比较, 以反义寡核苷酸处理的Daudi细胞ST6GalImRNA的表达下调, ST6GalI酶活性下降, 并导致细胞表面α2, 6 唾液酸水平降低。结论 反义寡核苷酸可有效降低Daudi细胞表面α2, 6 唾液酸化水平, 内源性减少α2, 6 唾液酸对Daudi细胞CD2 2受体的屏蔽作用Abstract: Objective To study the antisense oligonucleotides (antisense ODN) in down regulation of α2,6 sialyltransferase (ST6Gal I) activity and α2,6 sialylation of cell surface of Burkitt s lymphoma cell line Daudi. Methods Daudi cells were treated with modified antisense ODN in vitro, the ST6Gal I mRNA expression was examined by RT PCR, the ST6Gal I enzyme activity was measured with fluorometric sialyltransferase assay and the amount of α2,6 sialylation of cell surface was determined by FACS analysis.Resul...
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