癌胚抗原重组痘苗病毒转染树突状细胞诱导CEA特异性T细胞免疫

Induction of CEA-Specific cytotoxic T lymphocytes by dendritic cells transfected with CEA-recombinant vaccinia virus

摘要: 目的　研究人癌胚抗原重组痘苗病毒 (rV CEA)转染树突状细胞 (DC)在体外诱导CEA特异性的T细胞免疫。方法　将rV CEA转染外周血单个核细胞来源的DC后用于激发自体的T细胞, 通过对T细胞的增殖及杀伤功能的检测, 与野生型痘苗病毒 (V 76 1)转染的DC激发的T细胞进行比较。结果　经rV CEA转染的DC激活的T细胞增殖力强, 对CEA分泌性肿瘤细胞具特异性杀伤作用。结论　rV CEA转染的DC可以诱导CEA特异性T细胞活性。

Abstract: Objective To study the in vitro immune response induced by dendritic cells(DCs) transfected with CEA-recombinant vaccinia virus (rV-CEA). Methods DCs collected from peripheral blood monocytes were transfected with rV-CEA and then cocultured with autologous T cells.The proliferation of the T cells and their cytotoxic activity against CEA-secreting tumor cells were assessed and compared with those T cells induced by DCs transfected with wide type vaccinia virus (V-761). Results DCs transfected with rV-...