Abstract:
Objective To study the effects of Antisense gene to human NHE1 on the proliferation and apoptosis of SGC-7901 af ter t ransefection and it s potential role in the genetherapy for gast ric cancer. Methods NHE1 antisense gene eukaryotic expression vector const ructed was t ransfected into SGC-7901 with liposome DOTAP. We examined the changes of the proliferation index, the clone formation capacity in two layer soft agar, growth curve, apoptotic rates and nude mouse formation carcinoma capacity of the cells transfected antisense gene. Results Cells t ransfected NHE1 antisense gene compared with parent cells, Proliferation index decreased, the clone formation capacity in two layer soft agar play down, apoptotic rates increased, growth curve slowers. Format carcinoma capacity in nude mouse disappear. Conclusion Our study demonst rated that NHE1 antisense gene can rest rain gastric carcinoma proliferation and induce carcinoma cell apoptosis which implied that may be a new target gene for antisense gene therapy of gastric cancer.
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