Abstract: Objective 　To explore the differential expressed genes in lymphoma and reactive lymph node and performed an initial analysis on a novel gene. Hypethetical Protein FIJ39061, which is highly expressed in lymphnode of lymphoma. Methods 　The samples were collected and flash f rozen quickly to maintain the integrity of mRNA. The mRNA of the tissue from the lymphoma and reactive lymph node are marked with biotin respectively and hybridized with expression profile microarray, and the differential expressed genes are obtained. Initial bioinformatics analysis is performed on a novel gene named FLJ39061 which have no functional researches at present . Its gene structure, genomic localization, the physical and chemical characteristics of the putative protein, subcellular localization, functional domain and so on are predicted, and the systematic evolution analysis is performed on the similar proteins among several species. Results 　Using expression profile microarray, 152 differential expressed genes are uncovered. Efficient bioinformatics analysis have fundamentally identified that FLJ39061 is a nuclear protein, and probably is precursor or isomer of MAP kinase-activated protein kinase 2. Conclusion 　By bioinformatics analysis, FLJ39061 may involved in the phosphorylation regulation. It is anticipated that this project will advance our understanding of the molecular mechanisms in the neoplastic t ransformation of lymphoma and the new insights will help to identify promising molecular targets for therapeutic intervention.