脂氧合酶在胃癌中的表达及其意义
Expression and Its Signif icance of Lipoxygenase in Gastric Carcinoma
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摘要: 目的 检测5-脂氧合酶（5-lipoxygenase,5-LOX）和15-脂氧合酶-1（15-lipoxygenase-1,15-LOX-1）mRNA及蛋白在胃癌及癌旁正常组织中的表达,并研究两者间的关系。方法 采用RT-PCR和western blot检测胃癌组织及相匹配的癌旁正常组织中5-LOX、15-LOX-1mRNA和蛋白的表达。结果 5-LOX mRNA及蛋白在胃癌组织中的表达均显著高于癌旁正常组织（P〈0.05）,而15-LOX-1mRNA及蛋白在胃癌组织中的表达均显著低于癌旁正常组织（P〈0.05）。5-LOX与15-LOX-1在胃癌组织中的表达无明显相关（P〉0.05）。结论 胃癌组织中存在5-LOX和15-LOX-1的表达失衡,且可能与胃癌的发生发展有关,但二者之间无相关性。Abstract: Objective To test the expression of 5-lipoxygenase and 15-lipoxygenase-1 (15-LOX-1) mRNA and protein in gast ric carcinoma and adjacent normal tissue, and to investigate their relationship. Methods The 5-LOX and 15-LOX-1 mRNA and protein level were detected in pairs of gastric carcinoma and adjacent normal tissue by reverse transcription polymerase chain reaction (RT-PCR) and western blot methods. Results In gastric carcinoma, the expression of 5-LOX mRNA and protein was significantly higher than that in the adjacent normal tissue ( P < 0. 05), while the expression of 15-LOX-1 in gastric carcinoma was significantly lower than that in the adjacent normal tissue ( P < 0. 05) . No significant correlation was found between 5-LOX and 15-LOX-1 ( P > 0. 05) . Conclusion In gastric carcinoma, the expression of 5-LOX and 15-LOX-1 lost their balance, which was possibly correlated to the genesis and progress of gastric carcinoma. There was with close correlation between them.
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