鼻咽癌患者EBV感染与细胞免疫水平关系研究
Epstein-Barr Virus Infection and Its Correlation to Changes of Cell Immunity in Nasopharyngeal Carcinoma
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摘要: 目的 探讨鼻咽癌患者EBV感染与细胞免疫水平变化的相关性. 方法 采用PCR技术和流式细胞术（FCM）,检测55例鼻咽癌患者EBV感染状况和细胞免疫水平,分析二者的相关性,并与40例健康体检者对比分析.结果鼻咽癌患者外周血CD3＋细胞、CD4＋细胞明显减少,CD4＋/CD8＋比值明显下降,NK、CIK细胞占外周血淋巴细胞总数的百分比明显增高,与正常组比较差异有显著性（P〈0.01）;EBV阳性患者与阴性患者比较,CD3＋细胞、CD4＋细胞、CD4＋/CD8＋比值有明显差异（P〈0.01）,而NK细胞、CIK细胞占外周血淋巴细胞总数的百分比无明显差异（P〉0.05）.结论 EBV感染进一步抑制NPC患者细胞免疫功能,使病情进一步发展.监测EBV感染状况和细胞免疫水平,对鼻咽癌早期诊断、病情进展判断具有积极的临床意义.Abstract: Objective To explore the correlation of EBV infection to changes of cell immunity in Nasopharyngeal Carcinoma (NPC) . Methods EBV infection of 55 patient s with NPC was detected by PCR. T lymphocyte, N K and CIK cell activities were measured by flow cytometry. 40 normal healthy volunteers served as control. Results The mean value of T lymphocyte cell of NPC patient s was significantly lower than that of cont rol group ( P < 0. 01) ; Infection of EBV was significantly correlated to T lymphocyte cell activities in NPC ( P < 0. 01), but not correlated to CIK and N K cell activities ( P > 0. 05) . Conclusion EBV may inhibit cell immunity and participated in development of NPC. Detecting both EBV and changes of cell immunity can be potentially useful in early diagnosis and selection of a poor prognostic subgroup of NPC patients.
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