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放射线照射后NK-92 细胞对人宫颈癌细胞体外杀伤活性的研究

许恬怡, 凌 斌, 陈 纲, 周 颖, 王群华, 姚凤球, 陈峥峥, 赵卫东, 田志刚

许恬怡, 凌 斌, 陈 纲, 周 颖, 王群华, 姚凤球, 陈峥峥, 赵卫东, 田志刚. 放射线照射后NK-92 细胞对人宫颈癌细胞体外杀伤活性的研究[J]. 肿瘤防治研究, 2005, 32(06): 357-359. DOI: 10.3971/j.issn.1000-8578.645
引用本文: 许恬怡, 凌 斌, 陈 纲, 周 颖, 王群华, 姚凤球, 陈峥峥, 赵卫东, 田志刚. 放射线照射后NK-92 细胞对人宫颈癌细胞体外杀伤活性的研究[J]. 肿瘤防治研究, 2005, 32(06): 357-359. DOI: 10.3971/j.issn.1000-8578.645
XU Tian-yi, LING Bin, CHEN Gang, ZHOU Ying, WANG Qun-hua, YAO Feng-qiu, CHEN Zheng-zheng, ZHAO Wei-dong, TIAN Zhi-gang. In Vitro Cytotoxic Activity of Irradiated NK - 92 Cells against Human Cervical Cancer Cells[J]. Cancer Research on Prevention and Treatment, 2005, 32(06): 357-359. DOI: 10.3971/j.issn.1000-8578.645
Citation: XU Tian-yi, LING Bin, CHEN Gang, ZHOU Ying, WANG Qun-hua, YAO Feng-qiu, CHEN Zheng-zheng, ZHAO Wei-dong, TIAN Zhi-gang. In Vitro Cytotoxic Activity of Irradiated NK - 92 Cells against Human Cervical Cancer Cells[J]. Cancer Research on Prevention and Treatment, 2005, 32(06): 357-359. DOI: 10.3971/j.issn.1000-8578.645

放射线照射后NK-92 细胞对人宫颈癌细胞体外杀伤活性的研究

详细信息
    通讯作者:

    许恬怡

  • 中图分类号: R737. 33 ;R730. 55

In Vitro Cytotoxic Activity of Irradiated NK - 92 Cells against Human Cervical Cancer Cells

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    Corresponding author:

    XU Tian-yi

  • 摘要: 目的 研究放射线照射后NK-92细胞对人宫颈癌细胞的体外杀伤活性。方法 应用医用电子直线加速器对NK-92细胞进行照射处理(0、400cGy)后,以体外培养人宫颈癌细胞系Hela为靶细胞,以NK-92的敏感细胞株K562作为对照,应用4h^51Cr释放法检测不同效靶比情况下NK-92细胞的杀伤活性。结果 NK-92细胞0cGy照射组,效靶比较低时(1:1、5:1)对Hela细胞杀伤率为15%~33%,随效靶比升高,杀伤率随之上升,10:1时杀伤率显著上升为47%,20:1时杀伤率仅上升了3%;对于K562细胞,杀伤率为33%~69%。400cGy组照射后2d,NK-92细胞对Hela细胞不同效靶比的杀伤率与0cGy组相比稍有降低,其总体杀伤率为14%~47%,对K562细胞杀伤范围在30%~60%之间。结论 放射线照射后的NK-92细胞对人宫颈癌Hela细胞系具有一定的杀伤活性。

     

    Abstract: Objective  To study invitro cytotoxic activity of NK-92 cells against human cervical cancer cells and effect of irradiation on NK-92 cytotoxicity. Methods  NK-92 cells were mixed with 51Cr-labeled Hela or K562 cells at different effector-to-target ratios for a 4h 51Cr-release assay. On the other hand, NK-92 cells were irradiated at 0 and 400 cGy and tested after 2d for cytotoxicity against K562 and Hela cells. Results  NK292 cells (0 cGy) displayed high invitro cytotoxicity against Hela with a mean specific lysis of 47 % and 50 % at 10∶1 and 20∶1 E∶Tratios, while these levels at 1∶1 (15 %) and 5∶1 (33 %) E∶T ratios were lower ; The cytotoxicity of N K292 against K562 ranged from 33 % to 69 %. N K 3/ 92 cells maintained up to 14 %~47 % cytotoxicity against Hela and 30 %~60 % cytotoxicity against K562 when irradiated with 400cGy after 2d. Conclusion  In vit ro cytotoxic activity of NK-92 cells against Hela cells is high at suitable E∶T ratios, and substantial cytotoxicity can be retained up to irradiation dose of 400cGy.

     

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出版历程
  • 收稿日期:  2005-01-10
  • 修回日期:  2005-02-23
  • 刊出日期:  2005-06-04

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