Abstract: Objective 　To investigate the cell-mediated immunity enhancement effect of different adjuvants on the immunoprevention of poorly immunogenic murine melanoma vaccine. Methods 　Mice were immunized with inactivited D5 melanoma cells combined with five different adjuvant s ( FCA 、FCA + IL-2 +GM-CSF 、FIA + IL-2 + GM-CSF、FIA+SWZY、FIA + SWZY + IL-2 + GM-CSF) respectively and the control without adjuvants. 3 days after immunization, DTH response 、NK cell activity and specific CTL activity were assayed in half of the mice. The rest were inoculated with the live D5 tumor cells, 3 weeks after tumor inoculation, the same immunological parameters were measured. Results 　DTH response, NK cell activity and CTL activity of all the experimental groups were higher than that in the control group ( P < 0. 05), but decreased after the tumor grew larger. Conclusion 　Adjuvant FCA, FIA, IL-2, GM-CSF, SWZY could enhance the cell-mediated immune responses against poorly immunogenic tumor.The adjuvant SWZY could st rengthen immunoresponses similar to FCA, but had almost no harmful sideeffect, which might be a promising adjuvant for cancer vaccine.