姜黄素对膀胱癌细胞的p300 表达的影响

方　武; 李文威; 高淑华; 邓和鸣; 李香普

doi:10.3971/j.issn.1000-8578.580

姜黄素对膀胱癌细胞的p300 表达的影响

435001 湖北省黄石市第一医院泌尿外科

详细信息

通讯作者:
方　武

中图分类号: R737. 14
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出版历程
- 收稿日期: 2006-01-26
- 修回日期: 2006-07-26
- 刊出日期: 2007-02-04

The Effect of Curcumin on Expression of p300 in the Bladder Cancer T24 Cell

Department of Urology, First Hospital of Huangshi City, Huangshi 435001, China

More Information

Corresponding author:
FANG Wu

摘要

摘要: 目的了解姜黄素对p300表达的影响，探讨其抗膀胱癌的机制。方法用不同浓度的姜黄素作用膀胱癌细胞T24，MTT检测细胞增殖抑制率，RT-PCR法检测p300、c-fos、c-jun-的mRNA表达，蛋白免疫印迹法检测乙酰化组蛋白H3、H4、c-fos、叫un、p300蛋白表达。结果姜黄素能抑制T24细胞增殖，且具有量效关系和时效关系。姜黄素能抑制p300、c-fos、叫unmRNA和蛋白的表达，抑制组蛋白H3、H4的乙酰化，也具有量效关系。结论姜黄素可以抑制p300的表达，此可能是姜黄素抗膀胱癌的机制之一。
- 姜黄素 /
- p300 /
- c-fos /
- c-jun
Abstract: Objective 　To investigate the effect of curcumin on express of regulation p300 and mechanism of anticancer in vitro. Methods 　The bladder T24 cell was treated with curcumin in different concentration. The cell growth inhibition was determined with MTT, the level of p300 、c-fos、c-jun mRNA were assayed by reverse t ranscriptase polymerase chain reaction (RT-PCR), and the expression of acetyl histone H3 and H4 、p300 、c-fos、c-jun protein were evaluated by western blot . Results 　Curcumin could significantly inhibit proliferation of T24 cell in a dose and time-dependent manner. The expression of p300 、c-fos、c-jun mRNA and protein was totally decreased with increasing concent rations of curcumin. Curcumin can inhibit the expression of acetyl histone H3 and H4 protein in a dose-dependent manner. Conclusion 　Curcumin can inhibit the expression of p300, which may be it s molecular mechanism of anticancer.
- Curcumin /
- p300 /
- c-fos /
- c-jun