高级搜索
应用蛋白质组学方法研究大肠癌分化相关蛋白[J]. 肿瘤防治研究, 2007, 34(04): 253-255. DOI: 10.3971/j.issn.1000-8578.566
引用本文: 应用蛋白质组学方法研究大肠癌分化相关蛋白[J]. 肿瘤防治研究, 2007, 34(04): 253-255. DOI: 10.3971/j.issn.1000-8578.566
A Proteomic Study on Differences among High and LowDifferentiation Colorectal Carcinoma Tissues[J]. Cancer Research on Prevention and Treatment, 2007, 34(04): 253-255. DOI: 10.3971/j.issn.1000-8578.566
Citation: A Proteomic Study on Differences among High and LowDifferentiation Colorectal Carcinoma Tissues[J]. Cancer Research on Prevention and Treatment, 2007, 34(04): 253-255. DOI: 10.3971/j.issn.1000-8578.566

应用蛋白质组学方法研究大肠癌分化相关蛋白

A Proteomic Study on Differences among High and LowDifferentiation Colorectal Carcinoma Tissues

  • 摘要: 目的 研究人大肠癌高、低分化组织及癌旁正常粘膜之间蛋白质组表达差异,寻找和鉴定高、低分化癌组织之间差异表达蛋白。方法 收集新鲜大肠癌标本,根据病理诊断结果分为高分化癌组、低分化癌组,以癌旁正常肠粘膜作为对照。提取组织总蛋白,进行双向凝胶电泳,对得到凝胶图谱进行合成、对比和差异分析,比较和寻找高分化与低分化组织之间表达差异点。将这些差异蛋白点进行肽指纹图分析及网上数据库鉴定。应用免疫组织化学方法检测组织中蛋白质表达。结果 高分化和低分化癌标本中均存在钙网硬蛋白前体、O98007蛋白类似物的特异表达以及AAH10915、AAH75839蛋白表达上调;在高分化癌组中存在E980237蛋白的特异表达以及三磷酸异构酶、肝脂肪酸结合蛋白表达上调;在低分化癌组中存在2HHEB(氧化血红蛋白变异体)和锌指蛋白312类似物的特异表达以及Q9TQL5蛋白(Fragment)类似物表达上调。HSP27在大肠癌高分化组的表达率显著高于低分化组。结论 高分化癌组织与低分化癌组织之间存在蛋白质组差异表达,这些蛋白与大肠癌细胞分化有关。

     

    Abstract: Objective  To explore the proteomic differences among the high and low differentiation colorectal carcinoma tissues by using two-dimensional gel elect rophoresis (2-DE) and mass spectromet ry. Methods  In sample preparation, the fresh carcinoma tissue was cut off from the specimen and these tissues were classified into three groups according to the postoperative pathological diagnosis : high differentiation carcinoma, low differentiation carcinoma and normal epithelium. Protein separation was performed using 2-DE. After silver-staining, the images of the gels were scanned and analyzed to find the differentially expressed protein-spots in each group. We acquired the peptide mass fingerprintings ( PMF) of differentially expressed protein-spots by matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and identified the proteins by data searching in the Mascot-database. Protein expression was assayed by immunohistochenistry. Results  We detected calreticulin precursor and O98007-like protein expressed exclusively and AA H10915 和AAH75839 increased in both the two cancer groups. In high differentiation group, E980237 and tubulin beta chain expressed exclusively and triosephosphate isomerase and fatty acid-binding protein increased. In low differentiation group, 2 HHEB and Zinc finger protein312-like protein expressed exclusively and Q9 TQL5-like protein increased (over than 5 fold) . The HSP27 positive rate is significantly higher in high differentiation group than low differentiation group . Conclusion  Some differentially expressed proteins existed in high and low differentiation colorectal carcinoma as well as the normal colon mucosa. These proteins are related with colorectal carcinoma cell differentiation.

     

/

返回文章
返回