Abstract: Objective Through the analysis of the serum differentially expressed proteins of the health adults, the Non-Hodgkin s Lymphoma(NHL) with complete response(CR) after 6 cycles chemotherapy and the NHL without CR or recrudescence in 3 months after 6 cycles chemotherapy, the research is endeavored to provide a theoretical foundation for the serological index in screening, establishing the clinical therapeutic effect monitoring and prognosis evaluation of NHL. Methods The research applied SELDI-TOF MS and IMAC-u chip to detection the differentially expressed serum proteins of the health adult s, the N HL patient s with CR af ter six cycles chemotherapy and the N HL patient s with no remission or recrudescence in three months af ter six cycles chemotherapy who are 35 cases respectively, and to constitute the decision t ree classification model. Results 　(1) Between the health adult s and the NHL with CR af ter 6 cycles chemotherapy, six kinds of differentially expressed proteins were found to have the categorizing significance, whose M/ Z are M8 778. 14 u, M8 887. 83 u, M8 529. 39 u, M9 076. 76 u, M9 242. 75 u, M5 848. 92 u respectively, and the protein with the M/ Z M5 848. 92 u was selected by the sof tware system to establish the decision t ree classification model with the sensitivity of 88. 57 % and the specificity of 100 %. (2) Between the health adult s and the NHL without CR or recrudescence in 3 months af ter 6 cycles chemotherapy, six kinds of differentially expressed proteins were found to have the categorizing significance, whose M/ Z are M8 890. 26 u, M5 853. 70 u, M5 444. 38 u, M8 784. 58 u, M6 354. 26 u, M8 539. 28 u respectively, and the protein with the M/ Z M 5 444. 38 u was selected by the sof tware system to establish the decision t ree classification model with the sensitivity of 85. 71 %, and the specificity of 100 %. (3) Between the NHL with CR af ter 6 cycles chemotherapy and the N HL without CR or recrudescence in 3 months after 6 cycles chemotherapy, six kinds of differentially expressed proteins were found to have the categorizing significance, whose M/ Z are M6 436. 04 u, M5 509. 43 u, M6 633. 93 u, M14 047. 6 u, M7 769. 91 u, M6 009. 63 u respectively, and the protein with the M/ Z M6 436. 04 u was selected by the sof tware system to establish the decision t ree classification model with the sensitivity of 77. 14 %, and the specificity of 100 %. Conclusion 　These 18 differentially expressed proteins which hold categorizing significance might potentially be a group of biomarkers for monitoring clinical effect and directing clinical t reatment of N HL.