舒林酸、尼美舒利对人胃癌SGC-7901 细胞增殖及COX-2 、NF-κB 表达的抑制作用
Inhibitory Effects of Sulindac and Nimesulide on Cell Proliferation and COX-2, NF-κB Expression in Human Gastric Carcinoma Cell Line SGC-7901
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摘要: 目的 探讨舒林酸、尼美舒利对人胃癌SGC-7901细胞增殖及COX-2、NF-κB表达的影响. 方法 对人胃癌SGC-7901细胞采用细胞培养,MTT法检测不同浓度舒林酸、尼美舒利对人胃癌SGC-7901细胞增殖抑制作用,免疫组化、western blot方法检测药物对COX-2、NF-κB表达的影响. 结果 舒林酸、尼美舒利可以抑制人胃癌SGC-7901细胞生长并具有浓度、时间依赖性;与阴性对照组相比,药物作用48h后尼美舒利100、200μmol/L组,舒林酸0.6、1.2mmol/L组COX-2、NF-κB蛋白表达明显减弱并具有剂量依赖性（P＜0.05）,COX-2、NF-κB之间正相关（P＜0.05）. 结论 舒林酸、尼美舒利可以抑制人胃癌SGC-7901细胞增殖,抑制NF-κB, COX-2的蛋白表达在二者抑制肿瘤机制中可能具有一定作用.Abstract: Objective To investigate the inhibitory effects of sulindac and nimesulide on cell proliferation and COX-2, NF-κB expression in human gast ric carcinoma cells. Methods SGC-7901 cells were treated by sulindac and nimesulide. The anti-proliferative effect was measured by using MTT assay ;COX-2 and NF-κB protein expression were examined by immunohistochemical stain and western-blot analysis. Results Tumor cells proliferation was inhibited in dose and time dependent manner. The expression of COX-2, NF-κB in 100, 200μmol/ L nimesulide groups and 0. 6, 1. 2mmol/ L Sulindac groups were significantly decreased than the negative group ( P < 0. 05) . There was positive correlation between COX22 and NF2κB protein expression ( P < 0. 05) . Conclusion The proliferation of SGC-7901 cells was inhibited after treated by sulindac and nimesulide. Suppressing COX-2, NF-κB expression may play some roles in the antitumor process of these two drugs.
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