RhoC 过表达对人脐静脉内皮细胞体外迁移 及血管生成能力的影响

RhoC 过表达对人脐静脉内皮细胞体外迁移及血管生成能力的影响

摘要: 目的　探讨RhoC 基因过量表达对内皮细胞体外迁移及血管生成过程的影响。方法　构建RhoC 真核表达载体,以脂质体转染人脐静脉内皮细胞( HUVE), 采用RT2PCR 和Western blot 检测RhoC 的mRNA 及蛋白表达水平; 划痕实验检测细胞迁移能力; 胶原基质胶三维培养检测内皮细胞体外血管生成能力。结果　转染RhoC 实验组与空载体对照组比较,RhoC mRNA 和蛋白表达明显增高; 细胞迁移能力和血管样结构生成能力明显增强。结论　RhoC 高表达能促进人脐静脉内皮细胞体外迁移及血管生成。

Abstract: Objective 　To investigate the effect s of RhoC overexpression on human umbilical vein endothe2 lial ( HUVE) cell migration and angiogenesis in vi t ro. Methods 　Transfecting RhoC gene into HUVE cells by Lipofectamine22000. The expression of RhoC mRNA and protein were detected respectively by RT2 PCR and Western blot, respectively. Wound assay in vi t ro and three2dimensional culture were used to de2 tect the migration and angiogenesis capacity of HUVE. Results 　RhoC expression was increased by t rans2 fecting RhoC gene into HUVE, meanwhile, the cells migration and angiogenesis capacity were enhanced. Conclusion 　RhoC may increase the capacity of HUVE migration and angiogenesis in vi t ro.