Abstract: Objective 　To investigate the apoptosis-inducing activity of Lobaplatin (Lob) on cisplatin-resistant human ovarian carcinoma cell line SKOV3/ DDP and it s related mechanism. Methods 　Cell proliferation in different concent ration and time was determined by MTT (Methyl thiazolyl tet razolium) assay, and compared with other parent cell SKOV3. Then light microscope and transmission elect ron microscope were used to examine the morphologic changes of SKOV3/ DDP cells. And cell cycles and apoptosis were examined by flow cytometry ( FCM) . Results 　The result of MTT colormetric assay showed that proliferation of SKOV3/ DDP cells was inhibited by lobaplatin in a dose-time-dependent fashion, and there was no significant difference ( P > 0. 05) in two cell lines. When SKOV3/ DDP cells were treated with lobaplatin at a certain concent ration, the morphologic changes were found by light, transmission electron microscope and flow cytomet ric analysis. Flow cytomet ric analysis showed that af ter using lobaplatin, cell cycle distribution changed at first and as the time prolonged apoptotic peak went higher. Conclusion 　Lobaplatin inhibit s proliferation of SKOV3/ DDP cells by inducing apoptosis and G0 / G1phase arrest may be involved in the mechanism.