As_2O_3对胃癌细胞SGC7901/ADR阿霉素耐药性逆转作用

Arsenic Trioxide Reversing the Multidrug Resistance of Gastric Carcinoma Cell Line SGC7901/ADR to Adriamycin

摘要: 目的探讨三氧化二砷(As2O3)对胃癌细胞SGC7901/ADR阿霉素(ADM)耐药性的逆转作用和对Pgp、GSTπ表达的影响。方法以胃癌多药耐药细胞株SGC7901/ADR为靶细胞,用MTT法检测SGC7901/ADR细胞对ADM的敏感性,用流式细胞仪检测细胞内药物浓度及免疫组织化学法检测细胞Pgp、GSTπ表达。结果0.4～0.8μmol/LAs2O3对耐药细胞SGC7901/ADR无明显毒性(P>0.05)。As2O3可下调Pgp、GSTπ表达,提高SGC7901/ADR细胞内ADM浓度,增加SGC7901/ADR细胞对ADM的敏感性。结论As2O3能够抑制SGC7901/ADR细胞Pgp、GSTπ表达,增加细胞内ADM药物浓度而部分逆转其对ADM的耐药性。

Abstract: Objective To explore the reversal effect of Arsenic trioxide(As_ 2O_ 3) on multidrug resistance of gastric tumor cell line SGC7901/ADR to adriamycin(ADM)and the impact of As_ 2O_ 3 to the expressions of P-gp and GST-π in SGC7901/ADR cells. Methods Multidrug resistant human gastric carcinomas cell line SGC7901/ADR was used as target cells,the sensitivity of cell to adriamycin was assessed with MTT assay,the drug concentration in cells was detected with flow cytometry(FCM),and the impact of arsenic trioxide t...