胃癌腹膜高转移潜能细胞系的建立及其生物学特性
Establishment and Characterization of A Highly Peritoneal Dissemination of Human Gastric Carcinoma Cell Line
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摘要: 目的利用人胃癌细胞系(GC9811)在裸小鼠体内反复接种建立一株具有腹膜高转移潜能的胃癌细胞系(GC9811P),并对其生物学特性进行观察,为实验研究提供模型。方法采用胃癌细胞系(GC9811)在裸小鼠腹腔内反复接种,行体外培养腹膜转移灶筛选高转移亚系,绘制细胞生长曲线,光镜、电镜下观察细胞形态,利用流式细胞仪、染色体分析等方法,研究该高转移亚系的细胞周期、增殖、染色体核型等生物学特性。结果母本细胞系GC9811腹膜转移形成率为33.3%(3/10),而GC9811P腹膜转移形成率为100%。两种细胞系腹膜结节组织学形态大体相似。增殖速度较母系加快。细胞周期分析G1期53.5%、G2期12.5%、S期37.1%。且遗传学特性包括染色体形态仍为人类核型,众数维持在104～126之间,占70%。结论具有腹膜高转移的胃癌细胞系GC9811P的建立及裸小鼠体内实验模型,为研究胃癌腹膜转移机制及探索新的治疗途径提供了极为有用的工具。Abstract: Objective To screen human gastric carcinoma cell line with spontaneous highly peritoneal metastasis in nude mice and to provide a model for experimental study. Methods The human gastric carcinoma cell line GC9811(1×10 7) was inoculated intraperitoneally into nude mice. The peritoneal metastasis nodules had developed and harvested aseptically.GC9811-p_ 1 was obtained by cultured in vitro. Repeat-ing the same procedure four cycle of stepwise selection, we established the GC9811-P_ 4 cell line with highly meta...
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