结直肠癌中PTEN蛋白的表达

Expression of PTEN in Colorectal Carcinoma

摘要: 目的探讨结直肠癌中PTEN蛋白的表达及其与侵袭和转移的关系。方法应用免疫组化SP法检测67例结直肠癌中PTEN、Ecad、MMP2的表达情况,5例正常大肠黏膜作对照。结果PTEN蛋白表达定位于细胞质。与正常大肠黏膜相比,癌组织中PTEN表达明显降低,并随分化程度降低呈递减趋势;淋巴结转移组明显低于无淋巴结转移组;与浸润深度呈负相关。结直肠癌中PTEN表达与Ecad呈正相关,与MMP2呈负相关;淋巴结转移组PTEN、Ecad低表达且MMP2高表达的人数明显高于无淋巴结转移组。结论PTEN、Ecad低表达,MMP2高表达在结直肠癌的侵袭和转移过程中可能起协同作用,联合检测可为结直肠癌生物学行为判定和进一步的研究和治疗提供有效病理学依据。

Abstract: Objective To investigate the expression of PTEN in colorectal carcinoma and its relationship to invasion and metastasis. Methods SP immunohistochemical technique was used to assess the expression of PTEN, E-cad and MMP-2 in 67 cases of colorectal carcinoma. Five cases of normal colorectal tissues were served as control. Results The PTEN positive reaction was seen in cytoplasm. Compared with normal colorectal tissues, the expression of PTEN in the colorectal carcinoma was lower in both positive rate and inte...