姜黄素诱导人结肠癌细胞株SW480凋亡及其bcl-2表达相关性研究

Effect of Expression of bcl-2 and Apoptosis Induction by Curcumin in Colon Cancer Cell SW480

摘要: 目的探讨姜黄素对人结肠癌细胞株SW480诱导凋亡的影响及其作用机制。方法5～50μM姜黄素分别处理SW480细胞6～48h后,MTT法检测细胞的生长活性,流式细胞仪检测细胞周期,TUNEL法检测细胞凋亡,免疫组化法检测细胞内bcl2蛋白表达水平。结果MTT显示姜黄素对SW480细胞具有细胞毒作用,呈时间浓度依赖性。FCM结果显示细胞周期中G0/G1期细胞比例增加,S期、G2/M期比例下降。TUNEL法显示凋亡细胞数增加,免疫组化结果显示bcl2基因表达明显减弱。结论姜黄素能抑制结肠癌SW480细胞的增殖并诱导细胞凋亡。其机制可能与干扰细胞周期及下调bcl2基因表达有关。

Abstract: Objective To study the effect of curcumin on apopsis and bcl-2 expression in SW480 human colon cancer cells. Methods The inhibitive effect of curcumin on SW480 was measured with MTT. Cell cycle phase was analysised by flow cytometry. Apoptosis of SW480 was studied by TUNEL. The expression of bcl-2 gene was determined by immunohistochemical analysis. Results MTT test showed that curcumin was cytotoxic to cells .the number of the apoptosis cell line increased.FCM showed that the proprortion of G_ 0/G_ 1 phase...