血管窦内皮细胞改变在肝癌组织缺血再灌注损伤中的作用
The Effect of Sinusoidal Endothelial Cells Changes on the Injury of the Hepatocarcinoma Following Ischemia and Reperfusion
-
摘要: 目的研究肝癌组织缺血再灌注后血管窦内皮细胞(Sinusoidalendothelialcells,SEC)的损伤。方法兔肝脏注射VX2肿瘤组织混悬液,建立肝脏肿瘤和缺血再灌注模型。测定再灌注各时点组织中超氧化物歧化酶(SOD)和一氧化氮(NO)的含量;用HE和荧光脱氧核苷酸末端转移酶介导的dUTP缺口末端标记法(TUNEL)染色观察和比较两种组织中SEC的凋亡情况。结果缺血再灌注后癌组织中的SOD浓度下降显著,于再灌注1h即达最低水平(64.59±4.97),其后逐渐恢复但至再灌注7d仍低于再灌注前(121.12±6.88)。NO浓度在肝癌组织和正常肝组织均有下降。肝癌组织缺血再灌注后SEC凋亡细胞数量明显增加,至1d时达最高水平54(3.2)。至再灌注7d时阳性细胞仍多于缺血再灌注前33(4.9),其凋亡细胞数量多于正常肝组织。结论SEC的改变在肝癌组织缺血再灌注后的损伤过程中起重要作用。肝癌组织的改变较正常肝组织更为显著。Abstract: Objective To study the injury of the sinusoidal endothelial cells (SEC)in the hepatocarcinoma tissues following ischemia and reperfusion. Methods The hepatocarcinoma animal models were established by implantation of VX_ 2 tumor constitution mass into the liver of rabbits. The concentrations of Superoxide Dismutase (SOD) and Nitric Oxide(NO) were examined, and the apoptosis of SEC in the hepatocarcinoma and normal hepatic tissues were observed by means of HE staining and terminal deoxynucleotidyl transferase...
下载:
