熊果酸对A549细胞增殖、凋亡的影响及其机制
Effects of Ursolic Acid on Proliferation,Apoptosis of Human Non-small Cell Lung Cancer A549 Cells and Possible Mechanism
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摘要: 目的探讨三萜酸类化合物熊果酸(UrsolicAcid,UA)对人肺腺癌A549细胞增殖和凋亡的影响及其分子机制。方法利用噻唑蓝(MTT)比色法观察UA对A549细胞增殖的影响。流式细胞仪检测UA对细胞周期的影响。用Hoechst33258荧光染色观察凋亡细胞核形态变化。利用免疫印迹法(Western-blot法)检测细胞中活化型Caspase-3蛋白及NF-κB表达的变化。结果UA明显抑制A549细胞的生长(P<0.05),并呈时间和浓度依赖。流式细胞仪检测发现UA作用24和48h后A549细胞阻滞在G0/G1期(P均<0.05)。经UA处理后的实验组细胞呈现典型的凋亡核固缩表现,胞核呈致密浓染的颗粒状或块状荧光。胞质检测到活性Caspase-3蛋白的表达增强,并呈时间依赖,同时,NF-κB表达随时间延长而减弱。结论熊果酸在体外对A549细胞具有抗增殖和诱导凋亡的作用。核因子NF-κB参与肺癌细胞的增殖与凋亡调控,UA可通过抑制NF-κB活性,激活Caspase-3,从而诱导细胞的凋亡。Abstract: Objective To investigate the inhibitory and apoptotic effect and possible mechanism of ursolic acid(UA) on the proliferation of human non-small cell lung cancer A549 cells. Methods The effect of UA on the proliferation of A549 cells was measured by MTT method. Hoechst 33258 fluorescent dying was used to observe UA induced morphological changes. The cell cycle was observed with flow cytometry. The activated protein level of Caspase-3 and NF-κB was detected by Western blot. Results UA strongly inhibitied the ...
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