端粒酶反义RNA 对HL-60 细胞端粒酶活性及细胞增殖的影响
The Effect of Telomerase Antisense RNA on Telomerase Activity and Cell Proliferation of HL-60
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摘要: 目的 探讨端粒酶反义 RNA对 HL- 60细胞端粒酶活性及细胞增殖的影响。方法 用脂质体介导法将 p BBS2 1 2 - h TR(反义端粒酶 RNA真核表达载体 )导入人髓性白血病细胞系 HL-60中, 采用 TRAP法测定端粒酶活性, 细胞生长动力学检测, FCM分析等方法观察其对 HL- 60细胞的端粒酶活性及细胞增殖影响。结果 端粒酶反义 RNA能显著抑制 HL- 60细胞的端粒酶活性, 抑制 HL- 60细胞的增殖并诱导其凋亡。结论 以端粒酶反义 RNA抑制端粒酶活性是治疗白血病的潜在途径。Abstract: Objective To study the effect of telomerase antisense RNA on telomerase activity and cell proliferation of HL-60 cells. Methods By using lipofectin mediated DNA transfection, telomerase antisense RNA was successfully introduced into HL-60 cells. By means of TRAP 、dynamics of cell growth 、flucytometry the changes of HL-60 cells in aspect of telomerase activity, proliferation were detected . Results Telomerase antisense RNA can significantly inhibit the telomerase activity and the proliferation of HL-60 cells and induce the apoptosis of HL-60 cells. Conclusion It seems to be a new methods to treat leukemia by using telomerase antisense to inhibit telomerase activity and the proliferation of HL-60 cells. telomerase is a new latent target to treat leukemia.
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